The Mass Spectrometry Center's expertise and available applications in HDX-MS are outlined below.
Hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) is a powerful and versatile method for probing protein structure and dynamics. HDX-MS can be applied to large proteins and multiprotein assemblies and does not require mutagenesis or covalent modification of the target protein(s) with exogenous labels or probes. The Mass Spectrometry Center has two special purpose instruments configured specifically for HDX-MS measurements – a Waters Synapt G2 HDMS mass spectrometer coupled with a NanoAcquity-HDX UPLC, and a Waters Synapt G2S HDMS mass spectrometer equipped with ETD and coupled with a NanoAcquity-HDX UPLC.
Applications of HDX-MS include:
- Epitope mapping
- Identification of drug binding sites and drug induced conformational changes
- Protein-protein interactions
- Effects of mutations on protein structure and dynamics
- Effect of formulations on the structure or stability of biopharmaceuticals
- Study of protein misfolding and pathological protein conformations
- Effects of phosphorylation and other post translational modifications on protein structure and dynamics
The Mass Spectrometry Center is currently working collaboratively on HDX-MS projects with multiple groups both within the University System of Maryland and at outside institutions.